Maurice LaMarche described the Brain's voice as "sixty-five percent Orson Welles, and thirty-five percent Vincent Price".
In the opening credits, Brain is writing a complex formula about the Universe Explained (simplified), and the resulting answer is THX 1138 (1971), the title of the first film by George Lucas.
Pinky and the Brain's genes were spliced using the Acme Bagel Warmer and Gene Splicer.
The Brain's voice is based upon that of Orson Welles. The voice actor portraying Brain is Maurice LaMarche, who also portrayed Orson Welles by dubbing Vincent D'Onofrio's role as Orson Welles in the movie Ed Wood (1994).
Originated as a series of segments on Animaniacs (1993) before being spun-off to its own show. It was moved to the WB's primetime line-up, where it won an Emmy. Then it was moved back to the Kids WB line-up, where it was Pinky, Elmyra & the Brain (1998) adding the familiar human character from Tiny Toon Adventures (1990). After a few episodes, it was cancelled.